Home

Description

Search Catalogues

Browse catalogues

Collections

Prices

Guidelines
CELL LINES
PLANT CELL LINES
PLANT VIRUSES
PLASMIDS
PHAGES
MICROORGANISMS
GENOMIC LIBRARIES
PROCEDURES
CATALOGUE

Search Web Site

Contacts

FAQ

Site Map

LABORATORY PROCEDURES FOR PLANT CELL VIRUSES

REFERENCE NO.: PVC/1998/2.02


TITLE: QUALITY CONTROL CHECK


INTRODUCTION

The virus infected plant material sent to the collection is subjected to electron microscopical examination. This includes a check for purity and a preliminary identification. Infectivity of the virus is tested by transmission to suitable host plants.

PROCEDURE

1 Virus infected materials sent to the collection are tested for infectivity by transmission to propagation and indicator plants (PVC/1998/2.02 Appendix 2; PVC/1998/2.02 Appendix 3; PVC/1998/2.02 Appendix 4)

2 Virus infected materials sent to the collection for propagation and subsequent deposit are subjected to electron microscopy to test for purity and for preliminary identification. A tentative group designation based on particle morphology can be made - with few exceptions - for members of most plant virus genera. The method is detailed in PVC/1998/2.02 Appendix 5.

LIST OF GLASSHOUSE AND LABORATORY PROCEDURES

PVC/1998/2.02 Appendix 2 Mechanical transmission

PVC/1998/2.02 Appendix 3 Grafting of material to healthy host plants

PVC/1998/2.02 Appendix 4 Transmission of virus by insect vectors

PVC/1998/2.02 Appendix 5 Electron microscopy - Leaf dip assay


Guidelines prepared for CABBI by DSMZ, 3 Feb. 1998
Page Layput by CERDIC
Copyright CABRI, 1998  

© The CABRI Consortium 1999 - 2023
This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium.
Site maintained by Paolo Romano. Last revised on February 2023.