Guidelines
CELL LINES
PLANT CELL LINES
PLANT VIRUSES
PLASMIDS
PHAGES
MICROORGANISMS
GENOMIC LIBRARIES
PROCEDURES
CATALOGUE
LABORATORY PROCEDURES FOR MICROORGANISMS
Appendix
M/1998/2.05 Appendix
1
Selected tests used in the characterisation and quality control of bacteria Example Morphological and staining characteristics
|
Shape Arrangement Irregular forms Sheaths Budds Appendages |
Prosthecae Stalks Holdfasts Motility, swimming Motility, gliding Gram reaction |
Acid fastness Cell inclusions Flagella, number and insertion Size Ends Change during growth phase |
Cultural characteristics
|
Colonies Shape opacity Size consistency elevation emulsifiability edge surface |
Growth in broth Surface Turbidity deposit |
Physiological characteristics
|
Phototrophic Chemoautotrophic Chemoheterotrophic Aerobic growth Anaerobic growth Phage susceptibility |
Temperature range Optimum temperature vpH rangeNutrition Growth factors Serology |
Gaseous needs Resistance to antibiotics Growth on selective media Growth in presence of NaCl Heat resistance |
Biochemical characteristics
|
Metabolic pattern Specific enzyme tests Commercial identification kits |
Oxidative/fermentative utilisation of sugars |
Utilisation of substrates Production of specific metabolites |
Chemotaxonomical characteristics
|
Fatty acids, lipids Cell wall composition |
Ubi-/Menaquinones Polyamines
|
Protein pattern Hopanoids |
DNA techniques
|
Mol% G+C DNA hybridization |
RAPD AFLP |
RFLP Sequencing |
Guidelines prepared for CABRI by DSMZ, CBS and BCCM, 17 May 1998
Page layout by CERDIC
Copyright CABRI, 1998
© The CABRI Consortium 1999 -
2023
This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium.
Site maintained by Paolo Romano. Last revised on February 2023.